Prevalence of Cryptosporidium spp. and Enterocytozoon bieneusi in beef cattle in the Hebei Province of China

Cryptosporidium spp. and Enterocytozoon bieneusi (E. bieneusi) are two common opportunistic pathogens that can infect humans animals worldwide. The available data on the prevalence of these is insufficient in Hebei Province, China, which located Bohai Sea region. In present study, 718 fecal specimens native beef cattle from eight farms Province were examined for presence E. using nested PCR targeting small subunit (SSU) rRNA gene internal transcribed spacer (ITS) bieneusi. was 9.2% (66/718) 8.6% (62/718). C. andersoni (n = 56) parvum 10) identified this all parvum-positive belonged to IIdA20G1 gp60 gene. ITS sequence analysis obtained seven known genotypes, including J 48), BEB4 4), CHC8 3), EbpC 2), I D BEB6 1). Genotype zoonotic Group 1, while other genotype host-adapted 2. This first report occurrence Province. genotypes suggest be a potential source human or animal infection.